Acylated β-Caseins: Electrostatic Interactions and Aggregation

Electrostatic Modeling of Protein Aggregation

Ram Vanam M.S., Indiana University, December 2004. Electrostatic modeling of protein aggregation. Research Advisor: Paul L. Dubin Electrostatic modeling was done with Delphi of insight II to explain and predict protein aggregation, measured here for β-lactoglobulin and insulin using turbidimetry and stopped flow spectrophotometry. The initial rate of aggregation of β-Lactoglobulin was studied b...

DNA Packaging and Electrostatic Interactions

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Unfavorable electrostatic and steric interactions in DNA polymerase β E295K mutant interfere with the enzyme's pathway.

Mutations in DNA polymerase β (pol β) have been associated with approximately 30% of human tumors. The E295K mutation of pol β has been linked to gastric carcinoma via interference with base excision repair. To interpret the different behavior of E295K as compared to wild-type pol β in atomic and energetic detail, we resolve a binary crystal complex of E295K at 2.5 Å and apply transition path s...

Electrostatic Effects in Aggregation of Crystallin Proteins

Electrostatic Control of Spontaneous Vesicle Aggregation

Aqueous mixtures of cationic cetyltrimethylammonium p-toluenesulfonate (CTAT) and anionic sodium dodecylbenzenesulfonate (SDBS)spontaneously formequilibrium“catanionic”vesicleswhosechargedepends on the relative amounts of each surfactant. Aggregation of these vesicles was induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cro...